Device
Part:BBa_K137131:Design
Designed by: Josh Michener Group: iGEM08_Caltech (2008-10-23)
Lactose inducible lysis cassette expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Constitutive LacI expression ensures tight repression of lysis.
Source
Lysis cassette is cloned from Lambda Zap II genomic DNA.